Dataset
Plasma membranes isolated by unroofing - Grid 01
- Dataset ID:DS-10426
Release Date: 2025-01-08
Last Modified: 2025-01-08
Photo Caption: Central slab from a representative tomogram of a Homo sapiens sample.
Dataset Overview
Michalak, Sun, Sochacki et al. 2024 unroofing manuscript contains 10 grids. This is grid 1 of 10 and contains basal membranes of HSC3 cells. The cell line expresses EGFR-GFP as described in Panilla-Macua et al. Elife 2017 and has been serum starved. The cells were grown on Quantifoil Au 300 R2/2, 2 nm carbon grids prior to removing the apical membrane and cytoplasm with 0.7 bar of paraformaldehyde prior to vitrification.
Authors
- Willy W. Sun,
- Dennis J Michalak,
- Kem A. Sochacki,
- Jenny E. Hinshaw,
- Justin W. Taraska
Publications
Related Databases
EMDB ID:EMD-44921
Runs
38 of 38 Runs
Run Name | Tilt Series Quality Score | Objects | ||
|---|---|---|---|---|
 | tomo153 Run ID: RN-17090 | 5 - Excellent |
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 | tomo154 Run ID: RN-17091 | 5 - Excellent |
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 | tomo155 Run ID: RN-17092 | 5 - Excellent |
| |
 | tomo156 Run ID: RN-17093 | 5 - Excellent |
| |
 | tomo157 Run ID: RN-17094 | 5 - Excellent |
| |
 | tomo158 Run ID: RN-17095 | 5 - Excellent |
| |
 | tomo159 Run ID: RN-17096 | 5 - Excellent |
| |
 | tomo160 Run ID: RN-17097 | 5 - Excellent |
| |
 | tomo161 Run ID: RN-17098 | 5 - Excellent |
| |
 | tomo162 Run ID: RN-17099 | 5 - Excellent |
| |
 | tomo164 Run ID: RN-17100 | 5 - Excellent |
| |
 | tomo165 Run ID: RN-17101 | 5 - Excellent |
| |
 | tomo167 Run ID: RN-17102 | 5 - Excellent |
| |
 | tomo168 Run ID: RN-17103 | 5 - Excellent |
| |
 | tomo169 Run ID: RN-17104 | 5 - Excellent |
| |
 | tomo29 Run ID: RN-17105 | 5 - Excellent |
| |
 | tomo30 Run ID: RN-17106 | 5 - Excellent |
| |
 | tomo307 Run ID: RN-17107 | 5 - Excellent |
| |
 | tomo309 Run ID: RN-17108 | 5 - Excellent |
| |
 | tomo31 Run ID: RN-17109 | 5 - Excellent |
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