Dataset
Plasma membranes isolated by unroofing - Grid 02
- Dataset ID:DS-10427
Release Date: 2025-01-08
Last Modified: 2025-01-08
Photo Caption: Central slab from a representative tomogram of a Homo sapiens sample.
Dataset Overview
Michalak, Sun, Sochacki et al. 2024 unroofing manuscript contains 10 grids. This is grid 2 of 10 and contains basal membranes of HSC3 cells. The cell line expresses EGFR-GFP as described in Panilla-Macua et al. Elife 2017 and has been serum starved. The cells were grown on Quantifoil Au 300 R2/2, 2 nm carbon grids prior to removing the apical membrane and cytoplasm with 0.7 bar of paraformaldehyde prior to vitrification.
Authors
- Willy W. Sun,
- Dennis J Michalak,
- Kem A. Sochacki,
- Jenny E. Hinshaw,
- Justin W. Taraska
Publications
Related Databases
EMDB ID:EMD-44921
Runs
22 of 22 Runs
Run Name | Tilt Series Quality Score | Annotated Objects | ||
|---|---|---|---|---|
 | tomo_c2c1_11 Run ID: RN-17247 | 5 - Excellent | -- | |
 | tomo_c2c1_21 Run ID: RN-17256 | 5 - Excellent | -- | |
 | tomo_c2c1_22 Run ID: RN-17261 | 5 - Excellent | -- | |
 | tomo_c2c1_23 Run ID: RN-17266 | 5 - Excellent | -- | |
 | tomo_c2c1_25 Run ID: RN-17271 | 5 - Excellent | -- | |
 | tomo_c2c1_26 Run ID: RN-17274 | 5 - Excellent | -- | |
 | tomo_c2c1_27 Run ID: RN-17279 | 5 - Excellent | -- | |
 | tomo_c2c1_28 Run ID: RN-17281 | 5 - Excellent | -- | |
 | tomo_c2c1_29 Run ID: RN-17285 | 5 - Excellent | -- | |
 | tomo_c2c1_3 Run ID: RN-17291 | 5 - Excellent | -- | |
 | tomo_c2c1_33 Run ID: RN-17295 | 5 - Excellent | -- | |
 | tomo_c2c1_34 Run ID: RN-17300 | 5 - Excellent | -- | |
 | tomo_c2c1_53 Run ID: RN-17306 | 5 - Excellent | -- | |
 | tomo_c2c1_54 Run ID: RN-17310 | 5 - Excellent | -- | |
 | tomo_c2c1_58 Run ID: RN-17314 | 5 - Excellent | -- | |
 | tomo_c2c1_59 Run ID: RN-17317 | 5 - Excellent | -- | |
 | tomo_c2c1_65 Run ID: RN-17322 | 5 - Excellent | -- | |
 | tomo_c2c1_66 Run ID: RN-17326 | 5 - Excellent | -- | |
 | tomo_c2c1_7 Run ID: RN-17330 | 5 - Excellent | -- | |
 | tomo_c2c1_73 Run ID: RN-17334 | 5 - Excellent | -- |
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