Dataset
Plasma membranes isolated by unroofing - Grid 08
- Dataset ID:DS-10433
Release Date: 2025-01-08
Last Modified: 2025-01-08
Photo Caption: Central slab from a representative tomogram of a Homo sapiens sample.
Dataset Overview
Michalak, Sun, Sochacki et al. 2024 unroofing manuscript contains 10 grids. This is grid 8 of 10 and contains apical membranes of HEK293 cells. The cells are expressing FerriTag and GFP-FKBP-LCa (clathrin light chain) off of plasmids. The cells were transfected and grown on coverslips coated with fibronectin overnight, then adhered to Quantifoil Au 300 R1.2/1.3 grids coated with poly-lysine prior to removing the basal membrane and cytoplasm with 0.7 bar of paraformaldehyde. Unroofing occurred immediately prior to vitrification.
Authors
- Willy W. Sun,
- Dennis J Michalak,
- Kem A. Sochacki,
- Jenny E. Hinshaw,
- Justin W. Taraska
Publications
Related Databases
EMDB ID:EMD-44921
Runs
20 of 20 Runs
Run Name | Tilt Series Quality Score | Objects | ||
|---|---|---|---|---|
 | ASB2_ts1 Run ID: RN-13880 | 5 - Excellent |
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 | ASB2_ts10 Run ID: RN-13895 | 5 - Excellent |
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 | ASB2_ts14 Run ID: RN-13912 | 5 - Excellent |
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 | ASB2_ts15 Run ID: RN-13930 | 5 - Excellent |
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 | ASB2_ts16 Run ID: RN-13945 | 5 - Excellent |
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 | ASB2_ts18 Run ID: RN-13967 | 5 - Excellent |
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 | ASB2_ts19 Run ID: RN-13989 | 5 - Excellent |
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 | ASB2_ts2 Run ID: RN-14007 | 5 - Excellent |
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 | ASB2_ts20 Run ID: RN-14028 | 5 - Excellent |
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 | ASB2_ts21 Run ID: RN-14046 | 5 - Excellent |
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 | ASB2_ts22 Run ID: RN-14068 | 5 - Excellent |
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 | ASB2_ts23 Run ID: RN-14086 | 5 - Excellent |
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 | ASB2_ts25 Run ID: RN-14109 | 5 - Excellent |
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 | ASB2_ts27 Run ID: RN-14128 | 5 - Excellent |
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 | ASB2_ts3 Run ID: RN-14148 | 5 - Excellent |
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 | ASB2_ts4 Run ID: RN-14171 | 5 - Excellent |
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 | ASB2_ts5 Run ID: RN-14197 | 5 - Excellent |
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 | ASB2_ts7 Run ID: RN-14219 | 5 - Excellent |
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 | ASB2_ts8 Run ID: RN-14240 | 5 - Excellent |
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 | ASB2_ts9 Run ID: RN-14262 | 5 - Excellent |
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