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Dataset

CryoET of near-minimal cells Mycoplasma mycoides JCVI-Syn3A for the development of subtomogram averaging pipelines

  • Dataset ID:DS-10442

Release Date: 2024-10-15

Last Modified: 2024-10-15

key visualization for CryoET of near-minimal cells Mycoplasma mycoides JCVI-Syn3A for the development of subtomogram averaging pipelines

Photo Caption: Central slab from a representative tomogram of a Mycoplasma mycoides subsp. capri str. GM12 sample with selected set of annotations.

Dataset Overview

Raw movie stacks, tilt series, alignments and cryo-eletron tomograms of synthetically reduced genome cells. JCVI-Syn3A has 493 genes (about half of the genes of the WT Mycoplasma Mycoides Capri GM12). Samples were prepared by plunge-freezing via Leica GP2. Contained data was collected on a Krios G4 using a Falcon 4i detector and SelctrisX energy filter. Tomograms were reconstructed using AreTomo3 v1.0.23. The deposition also includes locations of cytosolic ribosomes identified using slabpick and membrane segmenations created using membrain-seg.

Authors

Publications

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Runs

262 of 262 Runs

Run Name
Tilt Series Quality Score
Annotated Objects
key visualization for 24aug01b_Position_1
24aug01b_Position_1

Run ID: RN-16443

3 - Moderate
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_10
24aug01b_Position_10

Run ID: RN-16444

3 - Moderate
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_10_2
24aug01b_Position_10_2

Run ID: RN-16445

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_10_4
24aug01b_Position_10_4

Run ID: RN-16446

4 - Good
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_11_4
24aug01b_Position_11_4

Run ID: RN-16447

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_12
24aug01b_Position_12

Run ID: RN-16448

4 - Good
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_1_2
24aug01b_Position_1_2

Run ID: RN-16455

4 - Good
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_12_3
24aug01b_Position_12_3

Run ID: RN-16449

4 - Good
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_1_4
24aug01b_Position_1_4

Run ID: RN-16456

4 - Good
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_14_3
24aug01b_Position_14_3

Run ID: RN-16450

3 - Moderate
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_1_5
24aug01b_Position_1_5

Run ID: RN-16457

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_15_3
24aug01b_Position_15_3

Run ID: RN-16451

4 - Good
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_15_5
24aug01b_Position_15_5

Run ID: RN-16452

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_16_2
24aug01b_Position_16_2

Run ID: RN-16453

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_17
24aug01b_Position_17

Run ID: RN-16454

3 - Moderate
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_20
24aug01b_Position_20

Run ID: RN-16458

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_20_2
24aug01b_Position_20_2

Run ID: RN-16459

4 - Good
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_20_3
24aug01b_Position_20_3

Run ID: RN-16460

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_21
24aug01b_Position_21

Run ID: RN-16461

4 - Good
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_21_2
24aug01b_Position_21_2

Run ID: RN-16462

3 - Moderate
  • cytosolic ribosome
  • membrane
  • 1
  • 2
  • 3
  • 4
  • 5
  • 14