Deposition

In situ architecture of the polar tube invasion apparatus from microsporidian parasites

Deposition ID:CZCDP-10307

Release Date: 2024-07-29

Last Modified: 2024-07-24

key visualization for In situ architecture of the polar tube invasion apparatus from microsporidian parasites

Photo Caption: Central slab of 4 representative tomograms with selected sets of annotations.

Deposition Overview

This deposition contains three datasets of dormant microsporidian spores from Encephalitozoon intestinalis and Encephalitozoon hellem. The datasets contain raw frames, tilt series, tomogram reconstructions and segmentations. Samples were prepared using the waffle-method and cryo-FIB milled. A subset of runs contains manual and manually curated annotations of spore organelles.

Authors

Deposition Data

Annotations:78

Publications

DOI:10.1101/2024.07.13.603322

Related Databases

EMDB ID:EMD-45674

Methods Summary

Annotations	Method Type	Method Details	Method Links

Deposited Data

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Data Contents

Tilt SeriesAvailable

FramesAvailable

CTFNA

AlignmentAvailable

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78 of 78 Annotations

Annotation Name	Object Shape Type	Method Type	Deposited In
107polar tube Annotation ID: AN-30811 Ground Truth	SegmentationMask	Hybrid	Dataset: Cryo-ET dataset of dormant microporidian spores from Encephalitozoon hellem Run: tomo_0112
106polar tube Annotation ID: AN-30810 Ground Truth	SegmentationMask	Hybrid	Dataset: Cryo-ET dataset of dormant microporidian spores from Encephalitozoon hellem Run: tomo_0112
105polar tube Annotation ID: AN-30809 Ground Truth	SegmentationMask	Hybrid	Dataset: Cryo-ET dataset of dormant microporidian spores from Encephalitozoon hellem Run: tomo_0112
104polar tube Annotation ID: AN-30808 Ground Truth	SegmentationMask	Hybrid	Dataset: Cryo-ET dataset of dormant microporidian spores from Encephalitozoon hellem Run: tomo_0112
103polaroplast Annotation ID: AN-30807 Ground Truth	SegmentationMask	Hybrid	Dataset: Cryo-ET dataset of dormant microporidian spores from Encephalitozoon hellem Run: tomo_0112
102plasma membrane Annotation ID: AN-30806 Ground Truth	SegmentationMask	Hybrid	Dataset: Cryo-ET dataset of dormant microporidian spores from Encephalitozoon hellem Run: tomo_0112
101microsporidian-type endospore Annotation ID: AN-30805 Ground Truth	SegmentationMask	Hybrid	Dataset: Cryo-ET dataset of dormant microporidian spores from Encephalitozoon hellem Run: tomo_0112
100microsporidian-type exospore Annotation ID: AN-30804 Ground Truth	SegmentationMask	Hybrid	Dataset: Cryo-ET dataset of dormant microporidian spores from Encephalitozoon hellem Run: tomo_0112
107vesicle Annotation ID: AN-30803 Ground Truth	SegmentationMask	Hybrid	Dataset: Cryo-ET dataset of dormant microporidian spores from Encephalitozoon hellem Run: tomo_0110
106polar tube Annotation ID: AN-30802 Ground Truth	SegmentationMask	Hybrid	Dataset: Cryo-ET dataset of dormant microporidian spores from Encephalitozoon hellem Run: tomo_0110
105polar tube Annotation ID: AN-30801 Ground Truth	SegmentationMask	Hybrid	Dataset: Cryo-ET dataset of dormant microporidian spores from Encephalitozoon hellem Run: tomo_0110
104polar tube Annotation ID: AN-30800 Ground Truth	SegmentationMask	Hybrid	Dataset: Cryo-ET dataset of dormant microporidian spores from Encephalitozoon hellem Run: tomo_0110
103polaroplast Annotation ID: AN-30799 Ground Truth	SegmentationMask	Hybrid	Dataset: Cryo-ET dataset of dormant microporidian spores from Encephalitozoon hellem Run: tomo_0110
102plasma membrane Annotation ID: AN-30798 Ground Truth	SegmentationMask	Hybrid	Dataset: Cryo-ET dataset of dormant microporidian spores from Encephalitozoon hellem Run: tomo_0110
101microsporidian-type endospore Annotation ID: AN-30797 Ground Truth	SegmentationMask	Hybrid	Dataset: Cryo-ET dataset of dormant microporidian spores from Encephalitozoon hellem Run: tomo_0110
100microsporidian-type exospore Annotation ID: AN-30796 Ground Truth	SegmentationMask	Hybrid	Dataset: Cryo-ET dataset of dormant microporidian spores from Encephalitozoon hellem Run: tomo_0110
107polar tube Annotation ID: AN-30795 Ground Truth	SegmentationMask	Hybrid	Dataset: Cryo-ET dataset of dormant microporidian spores from Encephalitozoon hellem Run: tomo_0109
106polar tube Annotation ID: AN-30794 Ground Truth	SegmentationMask	Hybrid	Dataset: Cryo-ET dataset of dormant microporidian spores from Encephalitozoon hellem Run: tomo_0109
105polar tube Annotation ID: AN-30793 Ground Truth	SegmentationMask	Hybrid	Dataset: Cryo-ET dataset of dormant microporidian spores from Encephalitozoon hellem Run: tomo_0109
104polar tube Annotation ID: AN-30792 Ground Truth	SegmentationMask	Hybrid	Dataset: Cryo-ET dataset of dormant microporidian spores from Encephalitozoon hellem Run: tomo_0109