Deposition

Cryo-ET of isolated segments of synaptic terminals, synaptosomes, from rat hippocampi

Deposition ID:CZCDP-10313

Release Date: 2025-02-20

Last Modified: 2025-02-20

key visualization for Cryo-ET of isolated segments of synaptic terminals, synaptosomes, from rat hippocampi

Photo Caption: Central slab of 4 representative tomograms with selected sets of annotations.

Deposition Overview

CryoET tomograms of synaptosomes for the development of segmentation and subtomogram averaging pipelines for synaptic vesicle proteins, post-synaptic receptors, etc. Each dataset has a slightly different sample preparation conditions. The tomograms collectively have a combination of full synaptosomes, presynaptic terminals, and other co-purified organelles that are not synaptosomes such as mitochondria.

Authors

Deposition Data

Annotations:484

Publications

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Related Databases

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Methods Summary

Annotations	Method Type	Method Details	Method Links
484	Automated	Prediction using membrain-seg without rescaling and weights MemBrain_seg_v10_alpha.ckpt	Source Code:membrain-seg +1 more

Deposited Data

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Data Contents

Tilt SeriesAvailable

FramesAvailable

CTFAvailable

AlignmentAvailable

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484 of 484 Annotations

Annotation Name	Object Shape Type	Method Type	Deposited In
100membrane Annotation ID: AN-69884	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (200 mg, no centrifugation, Cu grid, low tilt range, 1:50)Run: 24sep26a_Position_20_2
100membrane Annotation ID: AN-69883	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (200 mg, no centrifugation, Cu grid, low tilt range, 1:50)Run: 24sep26a_Position_20
100membrane Annotation ID: AN-69882	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (200 mg, no centrifugation, Cu grid, low tilt range, 1:50)Run: 24sep26a_Position_2
100membrane Annotation ID: AN-69881	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (200 mg, no centrifugation, Cu grid, low tilt range, 1:50)Run: 24sep26a_Position_19
100membrane Annotation ID: AN-69880	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (200 mg, no centrifugation, Cu grid, low tilt range, 1:50)Run: 24sep26a_Position_18_2
100membrane Annotation ID: AN-69879	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (200 mg, no centrifugation, Cu grid, low tilt range, 1:50)Run: 24sep26a_Position_18
100membrane Annotation ID: AN-69878	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (200 mg, no centrifugation, Cu grid, low tilt range, 1:50)Run: 24sep26a_Position_17_4
100membrane Annotation ID: AN-69877	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (200 mg, no centrifugation, Cu grid, low tilt range, 1:50)Run: 24sep26a_Position_16_3
100membrane Annotation ID: AN-69876	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (200 mg, no centrifugation, Cu grid, low tilt range, 1:50)Run: 24sep26a_Position_16_2
100membrane Annotation ID: AN-69875	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (200 mg, no centrifugation, Cu grid, low tilt range, 1:50)Run: 24sep26a_Position_15_2
100membrane Annotation ID: AN-69874	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (200 mg, no centrifugation, Cu grid, low tilt range, 1:50)Run: 24sep26a_Position_15
100membrane Annotation ID: AN-69873	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (200 mg, no centrifugation, Cu grid, low tilt range, 1:50)Run: 24sep26a_Position_13_2
100membrane Annotation ID: AN-69872	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (200 mg, no centrifugation, Cu grid, low tilt range, 1:50)Run: 24sep26a_Position_12_3
100membrane Annotation ID: AN-69871	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (200 mg, no centrifugation, Cu grid, low tilt range, 1:50)Run: 24sep26a_Position_12
100membrane Annotation ID: AN-69870	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (200 mg, no centrifugation, Cu grid, low tilt range, 1:50)Run: 24sep26a_Position_11_2
100membrane Annotation ID: AN-69869	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (200 mg, no centrifugation, Cu grid, low tilt range, 1:50)Run: 24sep26a_Position_11
100membrane Annotation ID: AN-69868	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (200 mg, no centrifugation, Cu grid, low tilt range, 1:50)Run: 24sep26a_Position_10
100membrane Annotation ID: AN-69867	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (200 mg, no centrifugation, Cu grid, low tilt range, 1:50)Run: 24sep26a_Position_1
100membrane Annotation ID: AN-69866	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (180 mg, 800 rpm, Au grid, high tilt range, 1:50)Run: 24oct15b_Position_7
100membrane Annotation ID: AN-69865	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (180 mg, 800 rpm, Au grid, high tilt range, 1:50)Run: 24oct15b_Position_6_2